Journal: Journal of ethnopharmacology

Article Title: Anemarrhena asphodeloides Bunge and Phellodendri Chinensis Cortex inhibits the PTGS2/EP2/cAMP/Epac1 signaling pathway to reduce microglial M1 polarization, thereby blocking chronic stress-induced depression-like behavior.

doi: 10.1016/j.jep.2025.119792

Figure Lengend Snippet: Fig. 8. Neomangiferin alleviates the release of proinflammatory factors in BV2 cells by inhibiting PTGS2/EP2/cAMP/Epac1 signals. (A) representative immunofluorescence staining of EP2 and Epac1, and (B) fluorescence area (n = 3). (C–D) Protein expression changes of EP2 and Epac1 (n = 3). The contents of PGE2 and cAMP in BV2 cells were determined by E-F Elisa kit (n = 3). (G) Representative images of zebrafish embryos in Neomangiferin experiment (n = 10). Data are expressed as mean±SD. *** means P < 0.001,** means P < 0.01,* means P < 0.05, ns means P > 0.05.

Article Snippet: Seal with 5 % skim milk powder at room temperature for 2 h. Primary antibody β-actin (rabbit, 1:5000; bs-00R,Bioss, China), GAPDH(rabbit, 1:10,000; 10494- 1-AP,Proteintech, China), HSP90(rabbit, 1:5000; 13171-1-AP,Proteintech, China), Arg-1 (rabbit, 1:1000; 16001-AP, Proteintech, China), iNOS (rabbit, 1:1000; ab283655, Abcam, UK), PTGS2 (mouse, 1:400; 66351-1-Ig, Proteintech, China), EP2 (rabbit, 1:1000; HA721380, HUABIO, China), EPAC1 (rabbit, 1:1000; A02483-3, Boster, China), incubated overnight at 4 ◦C.

Techniques: Immunofluorescence, Staining, Fluorescence, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Vaccines

Article Title: Protection and Alleviated Inflammation Induced by Virus-like Particle Vaccines Containing Plasmodium berghei MSP-8, MSP-9 and RAP1

doi: 10.3390/vaccines10020203

Figure Lengend Snippet: VLPs characterization. MSP-8 ( A ), MSP-9 ( B ) and RAP1 ( C ) VLP morphologies were visualized under TEM. MSP-8 ( D ), MSP-9 ( E ) and RAP1 ( F ) VLPs were loaded (8, 40 µg) for SDS-PAGE, and bands corresponding to each antigen were observed using either the polyclonal P. berghei antibody or the monoclonal M1 antibody.

Article Snippet: Nucleotide sequences of codon-optimized (RAP1) were obtained from GenScript, and compared with the original non-codon optimized RAP1 (XM_034565422.1) sequence acquired from NCBI database.

Techniques: SDS Page

Journal: Vaccines

Article Title: Protection and Alleviated Inflammation Induced by Virus-like Particle Vaccines Containing Plasmodium berghei MSP-8, MSP-9 and RAP1

doi: 10.3390/vaccines10020203

Figure Lengend Snippet: Experimental schedule. Mice were immunized twice with the combined VLP vaccines expressing MSP-8, MSP-9, and RAP1 of P. berghei at 4 weeks intervals, and blood samples were drawn 3 weeks after each immunization. Challenge infection was performed 4 weeks after boost immunization and half of the mice in each group were sacrificed at 1 week post-challenge.

Article Snippet: Nucleotide sequences of codon-optimized (RAP1) were obtained from GenScript, and compared with the original non-codon optimized RAP1 (XM_034565422.1) sequence acquired from NCBI database.

Techniques: Vaccines, Expressing, Infection

Journal: Vaccines

Article Title: Protection and Alleviated Inflammation Induced by Virus-like Particle Vaccines Containing Plasmodium berghei MSP-8, MSP-9 and RAP1

doi: 10.3390/vaccines10020203

Figure Lengend Snippet: IgG antibody response. Mice were intramuscularly immunized twice with the mixture of MSP-8, MSP-9 and RAP1 VLPs. Sera ( n = 6) were collected at regular intervals after each immunization with the VLPs. VLPs immunization induced IgG antibodies reacted with P. berghei Ag ( A ) and VLPs ( B ) upon prime and boost compared to naïve sera (** P < 0.01, *** P < 0.001).

Article Snippet: Nucleotide sequences of codon-optimized (RAP1) were obtained from GenScript, and compared with the original non-codon optimized RAP1 (XM_034565422.1) sequence acquired from NCBI database.

Techniques: